explain the roles of restriction endonucleases, DNA ligase, plasmids, DNA polymerase and reverse transcriptase in the transfer of a gene into an organism

explain the advantages of using recombinant human proteins to treat disease, using the examples insulin, factor VIII and adenosine deaminase

describe how the information in DNA is used during transcription and translation to construct polypeptides, including the roles of: • RNA polymerase • messenger RNA (mRNA) • codons • transfer RNA (tRNA) • anticodons • ribosomes