recognise organelles and other cell structures found in eukaryotic cells and outline their structures and functions, limited to: • cell surface membrane • nucleus, nuclear envelope and nucleolus • rough endoplasmic reticulum • smooth endoplasmic reticulum • Golgi body (Golgi apparatus or Golgi complex) • mitochondria (including the presence of small circular DNA) • ribosomes (80S in the cytoplasm and 70S in chloroplasts and mitochondria) • lysosomes • centrioles and microtubules • cilia • microvilli • chloroplasts (including the presence of small circular DNA) • cell wall • plasmodesmata • large permanent vacuole and tonoplast of plant cells
describe the semi-conservative replication of DNA during the S phase of the cell cycle, including: • the roles of DNA polymerase and DNA ligase (knowledge of other enzymes in DNA replication in cells and different types of DNA polymerase is not expected) • the differences between leading strand and lagging strand replication as a consequence of DNA polymerase adding nucleotides only in a 5’ to 3’ direction