9700_m21_qp_22
A paper of Biology, 9700
Questions:
6
Year:
2021
Paper:
2
Variant:
2

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Starch molecules are the main storage molecules in many types of cereal grain, such as the grain of the barley plant. When the seed inside a barley grain germinates, genes coding for digestive enzymes are switched on. The enzymes that are synthesised catalyse the hydrolysis of storage molecules such as proteins and starch. Explain what is meant by a gene. The hydrolysis of proteins in the barley seed produces amino acids that can be used in the synthesis of the proteins required for formation of the seedling (young plant). is an incomplete diagram of the molecular structure of the smallest amino acid, glycine. Each molecule of glycine has two carbon atoms. H C C H Complete to show the molecular structure of glycine. Starch is a mixture of two different molecules. Name these two molecules. Two of the enzymes synthesised by the barley seed are α-amylase and maltase. These are involved in the hydrolysis of the stored starch during seedling formation. In the food industry, the starch extracted from barley seeds (barley starch) is used in the production of sugar syrups. summarises the reactions catalysed by α-amylase in the production of maltose syrup and by maltase in the production of glucose syrup. barley starch maltose maltose glucose α-amylase maltase Some of the substances shown in are listed in Table 2.1. Complete Table 2.1 to identify which of the terms polysaccharide, monosaccharide and macromolecule apply to each of the substances listed. Use a tick (3) if the term applies and a cross (7) if the term does not apply. Put a tick (3) or a cross (7) in every box. Table 2.1 substance polysaccharide monosaccharide macromolecule glucose maltase maltose starch When producing sugar syrups, there are advantages in using enzymes extracted from microorganisms. For example, some enzymes extracted from microorganisms are heat stable. Heat-stable enzymes are used to increase productivity because the reactions can be carried out at higher temperatures. Suggest one other advantage of using enzymes obtained from microorganisms, rather than enzymes extracted from barley seeds, in the production of sugar syrups. is a graph showing how the activity of a-amylase extracted from barley seeds changes as the temperature increases from 10 °C to 66 °C. temperature / °C percentage enzyme activity Explain the effect of temperature on the activity of α-amylase extracted from barley seeds, as shown in . Sketch on the curve that would be obtained using α-amylase enzyme that is heat stable.
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Using a light microscope at a magnification of ×400, it is possible to identify different types of blood cell in prepared slides of mammalian blood. is a key to identify different types of blood cell in prepared slides of mammalian blood. In , letters C, D, E and F represent four different types of blood cell. key 1a nucleus present go to 2 1b nucleus absent C 2a large rounded nucleus D 2b nucleus not rounded go to 3 3a nucleus is kidney shaped E 3b nucleus is lobed F Identify the cell types C, D, E and F in . C D E F Explain why blood taken from a person with an infectious disease may have a different number of white blood cells compared with blood taken from a healthy person. As part of a research project, a team of doctors and scientists carried out tests to investigate the effect of altitude on the ability of blood to carry oxygen. They assessed the changes in the oxygen-carrying ability of their own blood as they walked from 1530 m above sea level to 5700 m above sea level, over a period of 14 days. 5700 m above sea level is an extremely high altitude. Table 4.1 is a summary of some of their results. Table 4.1 day 1 at 1530 m day 14 at 5700 m percentage saturation of haemoglobin with oxygen in blood leaving the lungs volume of oxygen delivered to tissues by 100 cm3 of blood / cm3 19.3 19.2 Compare the results for day 1 with the results for day 14 in Table 4.1 and explain the results shown for day 14. The results of the research project have led to an improved understanding of diseases such as sickle cell anaemia. Explain why sickle cell anaemia is an example of a disease.
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In March 2019, a tropical cyclone in the south-west Indian Ocean caused widespread flooding in a number of countries. The flooding and the damage caused by the cyclone meant that many people were at serious risk of cholera. After the natural disaster occurred, many different areas reported outbreaks of cholera. Within a short time the disease had spread widely and large numbers of people were affected. Cholera is caused by a bacterial pathogen. Name the bacterial pathogen that causes cholera. Suggest and explain why the people affected by the cyclone were at serious risk of cholera. In addition to the standard treatment for cholera, antibiotics are recommended for people who are moderately ill or seriously ill with the disease. Doxycycline is one of the main antibiotics used for the treatment of cholera. Doxycycline enters the pathogen and binds to one of the subunits of the bacterial ribosome. This prevents growth and reproduction of the bacterial cell. Suggest and explain how binding of doxycycline to ribosomes stops growth of the bacterial cell. Penicillin, which used to be prescribed for the treatment of cholera, has a different mechanism of action to doxycycline. State which part of the bacterial cell is affected by the action of penicillin. Mozambique was one of the countries badly affected by the cyclone. As part of the effort to prevent a greater number of cases of cholera from occurring, two different approaches were taken. • Approximately 900 000 doses of the oral cholera vaccine were sent to Mozambique and a large-scale vaccination programme was organised. • Medical centres were set up in Mozambique to treat people with cholera. Suggest and explain how the two different approaches helped to prevent a greater number of cases of cholera from occurring.