9700_s23_qp_21
A paper of Biology, 9700
Questions:
6
Year:
2023
Paper:
2
Variant:
1

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shows some fruits of the grapevine, Vitis vinifera. Sucrose is transported in the phloem of the grapevine to the fruits. In the fruits, sucrose is hydrolysed by the enzyme sucrase, which is found in cell walls. The glucose and fructose produced by the hydrolysis of sucrose pass through membrane proteins, known as hexose transporters, into the cytoplasm of the fruit cells. State why membrane proteins are required for the movement of molecules, such as glucose, across cell surface membranes into cells. Researchers investigated one type of hexose transporter, known as VvHT1, which is found in the fruit cells of V. vinifera. They used a mutant strain of yeast that has very few hexose transporters in its cell surface membranes to investigate the properties of VvHT1. The researchers inserted molecules of VvHT1 into the cell surface membranes of the mutant strain of yeast. • Equal volumes of mutant yeast cells with VvHT1 were kept in eight different concentrations of glucose solution. • The rate of uptake of glucose by the yeast cells in each solution was determined. • All the solutions were kept at the same temperature and pH. The results are shown in . concentration of glucose / μmol dm–3 rate of uptake of glucose / μmol min–1 g–1 The researchers concluded that VvHT1 is responsible for the facilitated diffusion of glucose into the cells. Explain how the results in provide evidence to support this conclusion. The researchers thought that grapevines could be modified to have more hexose transporters to increase the size and quality of grapes. Explain why increasing the number of hexose transporters could be commercially important to growers of grapevines. is a diagram of a protein in the cell surface membrane of a macrophage from a mouse. Macrophages use these proteins in antigen presentation. Non-self antigens bind to the proteins and are involved in the activation of specific T-lymphocytes during the immune response. antigen-binding region cytoplasm State what is meant by a non-self antigen. Some pathogens enter human cells. Macrophages partially digest these pathogens and present antigens to T-lymphocytes during immune responses. With reference to , explain how T-lymphocytes respond to infection by a specific type of pathogen.
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