0610_w12_qp_32
A paper of Biology, 0610
Questions:
6
Year:
2012
Paper:
3
Variant:
2

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For Examiner's Use The glucose concentration of the blood is maintained within the range 80 – 90 mg per 100 cm3 blood. is a flow chart showing how the body responds to changes in the glucose concentration of the blood after the absorption of a carbohydrate-rich meal and during strenuous exercise. change in blood glucose concentration detected by organ A blood glucose concentration increases above 90 mg per 100 cm3 large carbohydrate-rich meal followed by absorption of glucose from small intestine blood glucose concentration decreases below 80 mg per 100 cm3 organ A releases hormone B into the blood organ A releases hormone C into the blood blood glucose concentration decreases blood glucose concentration increases hormone B stimulates organ D to convert glucose to glycogen hormone C stimulates organ D to convert glycogen to glucose muscle contraction during strenuous exercise blood glucose concentration returns to 80 – 90 mg per 100 cm3 For Examiner's Use Name organ A and hormones B and C. A B C Name organ D that stores glucose as glycogen. Suggest why glucose is converted to glycogen rather than kept as glucose inside the cells. Name the type of control system used in homeostasis that returns the blood glucose concentration to 80 – 90 mg per 100 cm3. Animal hormones are used in the production of milk and meat. Bovine somatotrophin (BST) is used to increase milk production by cows. The hormone is produced by genetically modified bacteria that contain the cattle gene for making BST. It is used in the United States but is banned for use in the European Union. Outline how genes, such as the one for BST, are transferred from the cells of cattle to bacteria. For Examiner's Use Suggest advantages and disadvantages of using hormones, such as BST, in the production of milk and meat. advantages disadvantages
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For Examiner's Use Enzymes are biological catalysts. Define the term catalyst. Urease is an enzyme found in bacteria and in the seeds of some species of bean. The enzyme catalyses the reaction: urea + water carbon dioxide + ammonia The production of ammonia increases the pH of the area around the bacteria. The formation of ammonia can be used to study the progress of the reaction by testing the pH of the surrounding medium with a pH indicator, such as Universal Indicator solution. Some students carried out an investigation to find out if there was urease in the seeds of four different species of bean. • The germinating seeds were ground up in water and filtered to give an extract containing proteins. • Each extract was added to a urea solution and kept at 30 °C for 30 minutes (tubes 1 to 4). • Two more tubes (5 and 6) were included in the investigation. • Samples were taken from the reaction mixture at five-minute intervals and tested with Universal Indicator solution. The results are shown in Table 4.1. Table 4.1 presence of alkaline pH at intervals of 5 minutes test-tube bean species urea solution water soya yes no        mung yes no        jack yes no        broad yes no        soya no yes        no beans yes yes         = alkaline pH  = not alkaline pH For Examiner's Use Explain why the test-tubes were kept at 30 °C. Explain why test-tubes 5 and 6 were included in the investigation. State the conclusions that the students would make from the results of test-tubes 1 to 4. It is thought that some bean seeds produce ammonia as a protection against infection by microorganisms in the soil. Suggest what would happen to any ammonia that passes into the soil. For Examiner's Use Helicobacter pylori is a bacterium that infects the stomach and causes ulcers. The bacteria secrete urease that helps them to colonise the stomach lining. Explain why bacteria do not usually grow inside the stomach. Suggest how urease helps the bacteria to colonise the stomach. Explain how the immune system protects against infection by bacteria such as H. pylori.
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