5090_s24_qp_21
A paper of Biology, 5090
Questions:
6
Year:
2024
Paper:
2
Variant:
1

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Bacillus subtilis is a species of bacterium that is widely used in biotechnology. It can be used to manufacture the protein lipase for industrial use. is a diagram of B. subtilis. plasmid flagella D C B A Two of the parts labelled A, B, C and D in are involved in the manufacture of proteins such as lipase. Identify the two parts, name them and explain their role in protein manufacture. letter name role letter name role Suggest why B. subtilis has flagella. Explain why plasmids are useful in biotechnology. B. subtilis can be grown industrially in large containers. When B. subtilis is in ideal conditions it can reproduce rapidly, increasing the population size and the biomass of bacteria in the container. Graphs E and F in show how the biomass of B. subtilis increases after small populations of bacteria are added to two containers with different environmental conditions. 0.0 0.1 0.2 0.3 0.4 0.5 0.6 biomass of B. subtilis / g per dm3 time / hours F E Give the name of the large industrial containers used to grow bacteria. In graph E the biomass increases as shown in . Indicate using the letter M the part of graph E where bacteria are reproducing most rapidly. Calculate the percentage increase in biomass for graph E in the first 6 hours. Space for working. percentage increase in biomass = % Describe two differences between graph E and graph F. Suggest one environmental condition which could have caused the differences between graph E and graph F. Lipase is also produced by the human body. Explain why it is produced and how its function is different from the function of bile.
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