7.2. Transport mechanisms
A subsection of Biology, 9700, through 7. Transport in plants
Listing 10 of 310 questions
is a transmission electron micrograph of the bacterium that causes cholera, Vibrio cholerae. The flagellum shown in allows movement of the bacterium within the gut and may also function to help it to bind to an intestinal epithelial cell. The organism does not enter the cell but the toxin it releases can enter and cause damage. Large quantities of water, chloride ions and sodium ions are lost from the cell. flagellum magnification ×6000 People with symptoms of cholera have severe watery diarrhoea and as a result can become very dehydrated. Explain how a loss of chloride ions and sodium ions from the intestinal epithelial cell will cause a loss of water from the cell. The main treatment for cholera is oral rehydration therapy (ORT) using oral rehydration salts (ORS). This involves drinking a solution of electrolytes (mineral ions) and glucose. summarises the movement of glucose and sodium ions across an intestinal epithelial cell. includes three different types of cell surface membrane proteins: • SGLT1 is a cotransporter protein • GLUT2 and Na+/K+ pump are two types of carrier protein. water water glucose glucose glucose Na+ Na+ 3Na+ to capillary Na+ Na+ / K+ pump GLUT2 SGLT1 2K+ water water ADP ATP ORS lumen of intestine The first rehydrating solution developed for the treatment of cholera did not contain glucose. Using ORS, patients absorb a higher concentration of sodium ions and there is an increase in water uptake. With reference to : • outline the transport mechanisms that result in the movement of glucose and sodium ions across the intestinal epithelial cell • suggest how the movement of glucose and sodium ions increases water uptake by the cell. In severe cases of cholera, the rehydrating solution is given intravenously, as a drip directly into a vein. Suggest one reason why the rehydrating solution is given as a drip directly into a vein rather than into an artery. In very severe cases of cholera, antibiotics are also prescribed. The preferred antibiotic for treatment of cholera is a single dose of doxycycline, a form of tetracycline antibiotic. Tetracycline binds to a ribosomal subunit. Suggest what effect this will have on the metabolism of V. cholerae. A study was carried out to compare the effectiveness of the antibiotic tetracycline in the treatment of 118 patients with cholera. The patients were divided into four different treatment groups: • Group A, given one dose of 1 g tetracycline • Group B, given one dose of 2 g tetracycline • Group C, given a multiple dose (one dose of 500 mg tetracycline every 6 hours for 24 hours) • Group D, no antibiotic given. Following treatment, the volume of diarrhoea collected from each patient was measured every 16 hours for 128 hours. shows the mean volume collected for each group. time after treatment / h mean volume of diarrhoea / dm3 one dose of 1 g tetracycline Key one dose of 2 g tetracycline multiple dose (one dose of 500 mg tetracycline given every 6 hours for 24 hours) no antibiotic Describe the trends shown in . Explain whether the results support, or do not support, treating very severe cases of cholera with tetracycline. Some strains of V. cholerae are antibiotic resistant. Explain why this means that medical practitioners prefer to treat cholera with a single dose of antibiotic, rather than a multiple dose of the same antibiotic. Most people who have recovered from cholera rarely become ill again from the disease. In these people, antibodies have been identified that will bind either to the cholera toxin, or to the bacterial flagellum, or to the main bacterial cell. Explain why the antibodies are different, each one specific to its target. Evidence suggests that newborn babies of mothers who have had cholera have immunity to the disease. State precisely the type of immunity these babies are likely to have.
9700_w17_qp_22
THEORY
2017
Paper 2, Variant 2
Questions Discovered
310