investigate the progress of enzyme-catalysed reactions by measuring rates of formation of products using catalase and rates of disappearance of substrate using amylase
investigate and explain the effects of the following factors on the rate of enzyme-catalysed reactions: • temperature • pH (using buffer solutions) • enzyme concentration • substrate concentration • inhibitor concentration
describe and carry out investigations using redox indicators, including DCPIP and methylene blue, to determine the effects of temperature and substrate concentration on the rate of respiration of yeast