12.2. Respiration
A subsection of Biology, 9700, through 12. Energy and respiration
Listing 10 of 158 questions
The initial stages of respiration convert one molecule of glucose into two molecules of a 3C compound. State the name given to these initial stages where these stages occur in cells the total number of ATP molecules formed during these stages. Most of the ATP formed in respiration is produced within the mitochondria by oxidative phosphorylation. State the location, in the mitochondrion, of oxidative phosphorylation. Outline the process of oxidative phosphorylation. In an investigation, mammalian liver cells were homogenised (broken up) and the resulting homogenate centrifuged. Samples of the complete homogenate and samples containing only nuclei, only ribosomes, only mitochondria or only the remaining cytosol were incubated with: glucose pyruvate glucose and cyanide pyruvate and cyanide Cyanide inhibits oxidative phosphorylation. After incubation the presence or absence of carbon dioxide and lactate in each sample was determined. The results are summarised in Table 7.1. Table 7.1 samples of homogenate complete only nuclei only ribosomes only mitochondria only cytosol carbon dioxide lactate carbon dioxide lactate carbon dioxide lactate carbon dioxide lactate carbon dioxide lactate 1 glucose ✓ ✓ ✘ ✘ ✘ ✘ ✘ ✘ ✘ ✓ 2 pyruvate ✓ ✓ ✘ ✘ ✘ ✘ ✓ ✘ ✘ ✓ 3 glucose and cyanide ✘ ✓ ✘ ✘ ✘ ✘ ✘ ✘ ✘ ✓ 4 pyruvate and cyanide ✘ ✓ ✘ ✘ ✘ ✘ ✘ ✘ ✘ ✓ ✘ = absent ✓ = present With reference to Table 7.1, name the two organelles not involved in respiration. 1. 2. Explain why carbon dioxide is produced when mitochondria are incubated with pyruvate but not when they are incubated with glucose. Explain why, in the presence of cyanide, lactate is produced but carbon dioxide is not.
9700_s10_qp_41
THEORY
2010
Paper 4, Variant 1
Questions Discovered
158