9700_w13_qp_22
A paper of Biology, 9700
Questions:
6
Year:
2013
Paper:
2
Variant:
2

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For Examiner’s Use Keratin and chitin are two important biological molecules. Keratin is found in hair, fur and skin. Chitin is a modified polysaccharide found in a number of different organisms, for example in fungal cell walls and the hard outer skeletons of insects. Features of chitin and keratin are shown in the boxes in . example: Keratin and chitin are insoluble in water cellulose triglyceride amylopectin collagen Keratin and chitin have structural functions Keratin is a fibrous protein The monomers of chitin are β-1,4 linked Keratin and chitin contain nitrogen Write, in each box, the biological molecules from the list below that have the same feature. Each box may contain one, or more than one, biological molecule. The first box has been completed as an example. amylopectin cellulose collagen haemoglobin mRNA triglyceride For Examiner’s Use Chitin and the products of chitin hydrolysis have many useful medical and environmental applications. Chitinase enzymes can be used commercially to hydrolyse chitin. Enzyme stability and activity are important considerations in technological applications of chitinase. is a graph showing the effects of temperature on chitinase extracted from a soil bacterium. The relative activity of the enzyme was measured at different temperatures, with 100% representing maximum enzyme activity. relative activity / % temperature / °C With reference to , state the optimum temperature for the chitinase enzyme. For Examiner’s Use is a graph showing how temperature affects the stability of chitinase. The activity of the enzyme was measured over a time period of 72 hours at each of five different temperatures. relative activity / % time / h 28 °C 37 °C 40 °C 50 °C 60 °C With reference to and , describe and discuss the effect of temperature on chitinase activity and stability.
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