9700_s08_qp_4
A paper of Biology, 9700
Questions:
10
Year:
2008
Paper:
4
Variant:
0

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For Examiner’s Use shows the male and female flowers of maize. With reference to , describe how the flowering habit of maize encourages wind-pollination. In a maize plant, the anthers normally ripen before the stigmas are mature and ready to receive pollen. This encourages cross-pollination. Explain the potential advantages of cross-pollination to a plant species. For Examiner’s Use Cultivated maize is related to a wild Mexican grass, teosinte. Teosinte looks very different from maize. In particular, teosinte has a very hard layer surrounding the fruit, making it impossible to use as an edible grain. Archaeological excavations have found that maize in its edible form dates back at least 4000 years. It has been argued that the structural differences between teosinte and maize grains are so great that it is unlikely that maize could have been bred from teosinte so long ago. Investigations have been carried out into the genetic differences between teosinte and maize. A gene on chromosome 4, tga 1, containing 1042 base pairs, was found to be responsible for all of the structural differences between teosinte and maize. shows the DNA base sequence of the only part of tga 1 that always differs between teosinte and maize. teosinte GAT TGG GAT CTC AAG GCG GCG GGC GCG TGG maize GAT TGG GAT CTC AAC GCG GCG GGC GCG TGG Outline the principles of electrophoresis as used in sequencing this DNA. For Examiner’s Use Suggest how the difference in the base sequence of the tga 1 gene shown in could cause large differences in phenotype between teosinte and maize. With reference to , explain how these results support the suggestion that it would have been relatively easy for early farmers in Mexico to have bred maize from teosinte.
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For Examiner’s Use Outline the role of calcium ions in the transmission of nerve impulses. It has been suggested that during maturation of a sperm, the uptake of calcium ions is necessary to produce the vigorous movements of the sperm’s flagellum that allow it to penetrate the zona pellucida of an oocyte. Uptake of calcium ions can be measured by staining the sperm with a non-toxic fluorescent stain. Fluorescence increases as the concentration of calcium ions inside the sperm increases. Sperm from two types of mice were investigated: • wild-type mice, whose sperm have a particular protein, P. P is an ion channel found in the plasma (cell surface) membrane. • mutant mice whose sperm did not have protein P. The results of the investigation are shown in . sperm head sperm flagellum sperm from wild-type mice sperm from mutant mice mean percentage increase in fluorescence key: For Examiner’s Use With reference to , describe and explain the different mean percentage increases in fluorescence of sperm from wild-type and mutant mice, sperm heads and flagella. For Examiner’s Use The ability of sperm from wild-type and mutant mice to penetrate oocytes was tested using in-vitro fertilisation (IVF) of oocytes with and without a zona pellucida. The results are shown in . oocytes with zona pellucida oocytes without zona pellucida sperm from wild-type mice key: sperm from mutant mice percentage of oocytes penetrated by sperm Explain what is meant by in-vitro fertilisation. With reference to , explain the differences in the ability of sperm from wild- type and mutant mice to penetrate oocytes in IVF.
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