9700_s15_qp_42
A paper of Biology, 9700
Questions:
10
Year:
2015
Paper:
4
Variant:
2

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Intracytoplasmic sperm injection (ICSis a modification of the procedure for in-vitro fertilisation (IVF). A single sperm is injected into an oocyte rather than allowing one of many sperm to fertilise the oocyte. Suggest one problem that may arise from the use of ICSI. In preparation for ICSI, infertile women are injected at daily intervals with human follicle stimulating hormone (hFSH) to stimulate the growth and maturation of a number of ovarian follicles. Women may be treated with hFSH extracted from urine (u-hFSH) or with recombinant hFSH (r-hFSH) produced by genetically modified mammalian cells. Each molecule of hFSH consists of two different polypeptide chains, α and β. The genes for the α and β chains of hFSH, together with their promoters, have been inserted into mammalian ovary cells. Explain why promoters need to be transferred with desired genes when producing a genetically modified cell. After treatment with hFSH, oocytes are collected from mature ovarian follicles and examined to check that they have reached metaphase II of meiosis. Describe how an oocyte in metaphase I of meiosis can be distinguished from one in metaphase II of meiosis. You may use labelled diagrams to illustrate your answer. Two groups of women who were being prepared for ICSI were injected at daily intervals with either r-hFSH or with u-hFSH. The results of their treatment are shown in Table 2.1. Table 2.1 group of women receiving r-hFSH group of women receiving u-hFSH number of women total number of oocytes collected number of oocytes in metaphase II of meiosis With reference to Table 2.1, compare the effects of treatment with r-hFSH and u-hFSH and suggest an explanation for any differences. Whilst the two groups of women described in were being injected at daily intervals with FSH, the concentrations of oestrogen in their blood were measured. The results are shown in . days of FSH injection mean concentration of oestrogen / n mol dm–3 women injected with r-hFSH women injected with u-hFSH With reference to , compare the results for the two groups of women. Describe the role of oestrogen in the preparation of these women to receive an embryo.
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The Italian agile frog, Rana latastei, lives in woodlands in northern Italy. The adults breed by laying eggs in water in spring. The eggs hatch into tadpoles, which grow and develop for several weeks, before metamorphosing into adults and leaving the water. This must take place before cool weather arrives in autumn. shows an adult agile frog. This frog is now an endangered species. Many woodlands have been destroyed, leaving only isolated patches in which small populations of the frogs live. In order to try to prevent some of these small populations dying out completely, it has been suggested that tadpoles from larger populations could be introduced into the small populations, in order to increase genetic diversity. Suggest why increasing genetic diversity could help to conserve populations of Italian agile frogs. An argument against introducing individuals from one population into another is that there may be genetic differences between them that have evolved in response to exposure to different selection pressures. These genetic differences could be lost. An investigation was carried out into the time it takes for tadpoles to develop into frogs in two groups of populations: • populations living in the cool foothills of mountains • populations living in the warmer lowlands. Frogs, like all amphibians, are not able to control their body temperatures. In the wild, tadpoles in the foothills take about one month longer to develop into adult frogs than tadpoles in the lowlands. Suggest why tadpoles in the foothills take longer to develop into adults than tadpoles in the lowlands. The researchers collected eggs from foothill populations and from lowland populations, and kept them in identical conditions in the laboratory. They measured the masses of samples of the tadpoles until they metamorphosed into adult frogs. The results are shown in . The drop in mass towards the end of development shows when the tadpole changes into a frog. time / days tadpole mass / mg foothill populations lowland populations Key With reference to , describe the differences in the growth of tadpoles from foothill and lowland populations. Explain how the results shown in suggest that there are genetic differences between the foothill populations and the lowland populations of agile frogs. Suggest how these genetic differences may be important in increasing the chances of survival of the foothill populations in their natural habitat. With reference to the evidence from this investigation, explain why it may not be good conservation policy to introduce tadpoles of agile frogs from lowland populations to foothill populations.
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